POREM monitoring report (2020/12/31 – October-November-December 2020)

The work carried out for each POREM Action, in progress, was:

  1. Implementation actions

Action B.1: Pilot production of poultry manure based (POREM) bioactivator for soil bioremediation at pilot scale

Second cycle of POREM bioactivator production was completed in Italy, Spain and Czech Republic during third quarter of 2020.

In Italy, POREM pilot production was completed in Puglia, at Checchia Angiolina Farm in Biccari (FG) on November 2020 (Three piles of 3 tons). POREM bioactivator was used for II year application and horse bean harvesting (Action B.2). Temperature monitoring internal and environmental was carried out along the production cycle.

In Spain, POREM production was completed in Santomera (Murcia) (from August to November 2020). They were prepared 3 piles of 6 tons each. The POREM bioactivator produced was used for the II year of restoration tests in soils without vegetation and for a new area with almond planting.

Substrate sampling in Biccari and in Santomera was carried out in agreement with the new procedure, applied also in Czech and Spanish pilot productions:

  • Process sampling at t =30 days and 60 days, as well as in the proposal, with 2 samples for each pile (1 for crust-surface layer and 1 for core, inner part of pile),
  • Final POREM bioactivator sampling with 5 samples for each pile, AFTER MIXING, in order to describe quality of the product to be used for field test.

Referring to Calabria (IT) and Czech Rep., the description of the locations and the preparation of the piles was reported in the previous monitoring reports. They both had been completed within September 2020.

Virtual technical/organizational meeting between ASTRA and ENEA were performed respectively on November 6th, in order to define the best organization for POREM bioactivator use for field tests.


Action B.2: Degraded soil bioremediation in Spain, Italy and Czech with POREM bioactivator

Italy (ASTRA):

Emilia-Romagna Region:

The test on head cabbage (POREM Cycle II) started in mid-July 2020 and was completed. The test was set up on the same land and plot layout of the previous tomato test (POREM Cycle I). The POREM used in the test was produced (t=120 days cycle) at the Soldano poultry farm (project partner) in Calabria Region (Southern Italy). The post-harvesting soil samples were sent to CEBAS - CSIC for the analysis characterization. The crop harvest, final assessments, final crop and soil samplings were carried out in November 2020, as foreseen. 

Apulia (Puglia) Region:

The test on horse bean (POREM Cycle II) has just started in December 2020 and will go on up to June 2021. POREM bioactivator derives from PAV activated poultry dejections from litters of broiler addressed to EU organic production (Reg. 834/EC/2007; new reg. from 2021). The test will be executed on the same land and plot layout of the previous barley test (POREM Cycle I). As for the previous crop (barley in POREM Cycle I), ASTRA gave all indications for the initial soil sampling prior to POREM and commercial organic fertilizer application: two samples of soil per each treatment (one from the ‘upper’ land plot, bulk of the 3 replicates A-B-C; one from ‘lower’ plot land, bulk of 3 replicates D-E-F), for a total of 6 soil samples. They have been sent to CEBAS - CSIC for the analysis characterization. The first field assessments will occur at crop emergence.


Murcia Region (Santomera, Murcia):

  1. In the experimental field placed in Santomera, Murcia (Spain), where the POREM bioactivator was incorporated in a degraded area as a strategy to improve soil quality and health, MICRONADIR has continued monitoring the soils (spontaneous vegetation appeared, control of the plots, etc.). It is foreseen to add new doses of POREM bioactivator in January 2021 (first addition was in January 2020). Half of low dose plots 2020 received 125 tons per ha - 2021 will be received any other 125 tons per ha

At the end of the Project: Low dose plots applied once, High dose applied once ,High dose applied half 2020 half 2021

  1. Adding POREM for planting almond trees applying POREM bioactivator produced in 2020. A total of 12 plots have been prepared:
  • 6 Plots of VAP POREM ( 3 low dose: 125 tons per ha; 3 high dose: 250 tons per ha) 
  • 3 Plots of PM (poultry manure) (2 low dose: 125 tons per ha, 1 high dose: 250 tons per ha) 
  • 3 Plots No amended.

Almond trees will be planted in January, 2021.

All the soil samples were sent to the CEBAS-CSIC laboratory for their corresponding analysis.

Czech (EPS):

The winter crop test started in the Czech Republic. EPS test field is about 1 ha area, split in four flat-laying test plots (dimensions: 72m x 28m with 3 m division gaps). Location: Blatnička

October 10th, 2020: sowing of winter wheat with a seeder

October 29th, 2020: the first soil sampling after bioactivator POREM application

Winter crop harvesting and final soil sampling is planned on July 2021.

Action B.3: Modular unit production of POREM bioactivator for degraded soils in pre- industrial scale

Soldano received 4 offers related to the POREM bioactivator palletizing and packaging and on the basis of the best value for money (considering the transport costs, the offer flexibility, the work monitoring, etc.) Soldano has chosen the company MALDE LEGNAMI DI DI GIOSIO CIRO & C SAS in Salerno defining the process layout and realizing a pelletizing process video. Soldano has already produced some POREM pelletized samples and sent them to ASTRA and CSIC for analyses.

Action B.4: Training courses, workshops and guidelines for project replicability and transferability

On the basis of the COVID-19 problems the partners confirmed the following redefinition of the training course and workshop schedule:

  • POREM workshops
    • Italy:
      • ASTRA in May 2021
      • Soldano in June 2021
    • Spain: Micronadir: June 2021
    • Czech Republic: EPS in May 2021
  • POREM training courses
    • Italy:
      • ASTRA in June 2021
      • Soldano in June 2021
    • Spain: Micronadir in June 2021
    • Czech Republic: EPS in June 2021
  • 2 technical Guidelines
    • POREM production in May 2021
    • POREM field application in May 2021


Action B.5: POREM Business Plan

Astra started to asks for different offers about the external service related to the production of the POREM Business plan

  1. Monitoring of the impact of the project actions

Action C.1: Monitoring the low cost technologies used in soil restoration

Italy (ENEA):

ENEA has received the samples the following samples in these moths:

  • IT_C: t0, t120
  • IT_P: t0, t30 crust and core; pile 1, 2, 3
  • ES: t0; pile 1, 2, 3, 4=reference
  • CK: t0, t30 crust and core; pile 1, 2, 3

The samples were collectedè sent to Spain for treatmentè sent to ENEA for characterization

ENEA has received the samples at t0 and some at t30, collected from Spanish, Italian and Czech piles, belonging to the second POREM production cycle. Therefore, ENEA has started with the physico-chemical characterization, performed with:

- Thermogravimetric Analysis (TGA): to measure thermal stability and detect decomposition phases (ΔT and % mass loss) - X-ray diffractometric analysis (XRD): to detect mineralogical phases; - Scanning Electron Microscopy (SEM- EDS): to analyse morphological structure and carry out semiquantitative analysis.

As for the first production cycle, the search for inhibitors is performed by IZSLER, to prevent antibiotic risks.

Meanwhile, the global analysis on the characterization results of the first POREM production samples has allowed to draw up the summary documents which are the basis of the abstract presented for Ecomondo 2020 fair and the news published on some online magazine.

The Additional Staff, foreseen for action C1 and C2, has given his contribute on material characterization and related activities along the whole period.

Gas monitoring: The second POREM monitoring cycle will probably not start before Spring 2021 (èlimitation due to the pandemic measures è consequent extension of Action C1.2)

Spain (CSIC):

CSIC continued the chemical and biological characterization of all the POREM samples of Cycle II. CSIC concluded analyses of:

  • all samples from Calabria POREM production,
  • all samples from Czech POREM production,
  • all samples from Puglia
  • all samples from Santomera production,

with hundreds and hundreds of determinations. 

Sample preparations for ENEA physical characterization is in progress.

Action C.2: Monitoring of degraded soils

Italy (ASTRA):

Field and laboratory results from POREM Cycle II field tests, carried out by ASTRA on head cabbage in Cesena (Forlì-Cesena province; Emilia-Romagna region in Northern Italy) have been elaborated. In the meanwhile, the test on horse bean in Biccari (Foggia province, Apulia region in Southern Italy) is just started.

The cabbage harvest was executed at the end of October 2020. In these conditions, the test results between treatments were in line with the trend usually obtained in an optimal period.

The following results  were obtained with the available amount of 1 ton of POREM, that means ab. 40 units of N delivered with POREM in comparison to the 130 units of N with Standard Fertilizer as by IPM indication à saving of 69% N units in cabbage test with POREM.

  • Qualitative and productive results: The test proved that POREM delivered on cabbage achieved a yield statistically comparable to the yield obtained with a conventional mineral fertilizer (calculated on the basis of indications provided by the Product Specification for IPM of Emilia-Romagna region).
  • Plant observations: Overall trend of results confirmed the effectiveness of POREM particularly on vigor and NDVI, that caused a numerically higher development of plants, that were more leafy and covering; statistically comparable to the Standard Ref. fertilizer.
  • Plant observations: POREM give a certain “visual starter effect” on cabbage crop in all the parameters assessed. have been statistically analysed and presented to general meeting in Foggia on 3rd August 2020. The statistical variance among treatments was analysed with ANOVA and positive significative differences have been highlighted for some parameters. In order to complete the results, the quantity of fertilizer applied per hectare also in terms of Macroelements P and K, as already done for N, will be calculated and compared in the different tests.


Murcia Region (Santomera):

In the field plots controlled by MICRONADIR and placed in Santomera (Murcia, Spain), the comparison between the soil samples analysed at the beginning of the experiment and those from 6 months was made, on the basis of CEBAS-CSIC analysis. It is expected that there will be variations between both sampling times, since the soils must not yet be balanced, and the mineralization and / or humification processes must be developing. In addition, the possible vegetation that is spontaneously developing on the plots, although opportunistic and possibly with nitrophilic plants, may be contributing to the soils with some organic matter and it will undoubtedly affect some of the parameters that are being determined.

Also initial soil sampling of the new prepared area for almond test was carried out.

In addition, CEBAS-CSIC is also analysing all the soils that come from the different experiments carried out in Emilia-Romagna, in Apulia (Puglia), in Czech Rep. for a total of 100 samples with about 40 parameters each).

Czech Republic (EPS):

The EPS test field is located in an area characterized by intensive agriculture. The main factor leading to soil degradation in this area is incorrect agricultural practice, which has recently been accelerated by the climate changes (increase in the number of extreme events). There is a high level of compaction, lack of humus and nutrients in the soil and problems with infiltration and water retention. EPS does not own suitable land for the purpose of the POREM project. The selected EPS test field (Blatnička village cadaster near Uherské Hradiště) displays the character of degraded soil. Degradation features negatively affecting soil quality are:

  • degradation of physical properties of soil (structural damage, compaction, surface flow of water)
  • Undesirable changes in the content of nutrients in the soil (leaching, biological and abiotic immobilization)

Above discussed facts are supported by the publication “Monitoring of agricultural soils in the Czech Republic, 1992–2013 “published by Central Institute for Supervising and Testing in Agriculture.

The initial monitoring of the soil "before the application of POREM" was carried out just before the application of POREM on September 16th, 2020. The EPS sampled a total of 20 mixed samples of 0.5 kg each, 5 samples per test plot from the depth of 0-25 cm. For comparison purposes, one more sample was added and labeled as "CZ etalon" – from a slightly bushy strip of land located just adjacent to the field, representing the properties of non-degraded soil without intensive agricultural treatment.

October 29th, 2020: the first soil sampling after bioactivator POREM application – by an auger. 20 mixed samples, 5 samples per one field, 0.5 kg each sample were prepared and send to CEBAS.

The second soil sampling "after the POREM bioactivator application" is planned shortly after sowing the winter crops and final field preparation in late September – early October. Final soil sampling is expected after the July 2021.

Action C.3: Monitoring of socio-economic impact of the project and LCA

  • Astra has defined and prepared the questionnaire in English for monitoring of socio-economic impact
  • Astra has sent the questionnaire to EPS and Micronadir for translation, distribution and filling in from 120 farmers in Italy, Spain and Czech Republic. In particular:
  • Astra will be responsible to the POREM questionnaire distribution and filling in from 50 Italian farmer
  • Micronadir will be responsible to the POREM questionnaire distribution and filling in from 50 Spanish farmer
  • EPS will be responsible to the POREM questionnaire distribution and filling in from 20 Czech farmers
  • Astra will receive the filled questionnaire by ASTRA, EPS and Micronadir before the end of March 2021 and will carry out a statistical analysis of all the POREM questionnaire results

Action C.4: Performance indicators monitoring


  • During October and November 2020 monitoring, revising and update of POREM KPI following the indications and suggestion of project monitor
  • Predisposition of a new Deliverable “Update of the LIFE performance indicators table at the Midterm Report” following the EU indications, as attachment of the next Progress Report in January 2021.
  1. Public awareness and dissemination of results

Action D.1: Project dissemination plan: web-site, material, articles, Layman’s report and video

  • Updating of the project website and facebook page; at the end of November 2020:
    • Website visitors: 36,710
    • Facebook page: 90 friends
  • Production of 15 POREM articles.
  • 1 TV presentation
  • 1 Radio interview

Action D.2: Project dissemination plan: events, networking and contacts with Institutions and policy makers

On line participation at fair ECOMONDO-The Green Technologies Expo Rimini (Italy) 04/11/2020

Networking email contacts with the project manager of the following projects:

  • PPILOW project – INRA (FR) - Anne Collin - Organic and low-input outdoor farming solutions to improve welfare of poultry and pigs
  • AgriSpin project - L&F, SEGES (DK) - Heidi Hundrup – it seeks to find the answers to those questions and many more by identifying best practices for innovation and support systems in European agriculture
  • EURAKNOS project - Ghent University (B) - EURAKNOS wants to reinforce the EU agricultural knowledge base by building the blueprint for a datasystem to enable the farming/rural community easier access to best practices from all EU H2020 Thematic Networks
  • CropBooster-P project - Wageningen University (NL) - Judith van Veen - CropBooster-P will address this by identifying priorities and opportunities to adapting and boosting productivity to the environmental and societal changes
  • AgriLink project – INRA (FR) - Pierre Labarthe e Luke Lauprete - AgriLink aims to stimulate sustainability transitions in European agriculture through better understanding the roles played by farm advice in farmer decision-making
  1. Project management

Action E1: Project management

Progress global on line meeting held on 04/12/2020.

Monthly on line meeting between ASTRA, ENEA and CEBAS.